SNAI1 Polyclonal antibody

SNAI1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 26183-1-AP

Synonyms

Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

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Validation Data Gallery

• 

  WB analysis of HeLa using 26183-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 26183-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26183-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26183-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 26183-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26183-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 26183-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNAI1 antibody (26183-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 26183-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26183-1-AP (SNAI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, PC-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

26183-1-AP targets SNAI1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAI1 fusion protein Ag24248
• Full Name: snail homolog 1 (Drosophila)
• Calculated Molecular Weight: 264 aa, 29 kDa
• Observed Molecular Weight: 29-35 kDa
• GenBank Accession Number: BC012910
• Gene Symbol: SNAI1
• Gene ID (NCBI): 6615
• RRID: AB_2880415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; XPO1-Mediated EIF1AX Cytoplasmic Relocation Promotes Tumor Migration and Invasion in Endometrial Carcinoma; Authors - Yuhong Ye
human	WB	Aging (Albany NY); Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.; Authors - Ya Peng
human	IHC	Front Cell Dev Biol; Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis.; Authors - Rui Qian
human	WB	Oncotarget; AEG-1/MTDH-activated autophagy enhances human malignant glioma susceptibility to TGF-β1-triggered epithelial-mesenchymal transition.; Authors - Meijuan Zou
human	WB	J Cell Mol Med; Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis.; Authors - Jingjing Wu
human	WB	Cancer Manag Res; AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer.; Authors - Fen Xu

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