SMYD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMYD4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17594-1-AP

Synonyms

ZMYND21, SMYD 4, SET and MYND domain-containing protein 4, EC:2.1.1.-

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Validation Data Gallery

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  WB analysis of HepG2 using 17594-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17594-1-AP (SMYD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 17594-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human pancreas tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17594-1-AP targets SMYD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMYD4 fusion protein Ag11552
• Full Name: SET and MYND domain containing 4
• Calculated Molecular Weight: 804 aa, 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC035077
• Gene Symbol: SMYD4
• Gene ID (NCBI): 114826
• RRID: AB_10694429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMYD4, also named as SET and MYND domain-containing protein 4, is a 804 amino acid protein, which contains 1 MYND-type zinc finger and 1 SET domain. SMYD4 belongs to the class V-like SAM-binding methyltransferase superfamily. SMYD4, as a potential tumor suppressor, plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of Pdgfr-alpha, and could be a novel target for improving treatment of breast cancer. SMYD4 is a muscle-specific transcriptional modulator involved in development.

Publications

Species	Application	Title
zebrafish,human	WB,IF,IHC,CoIP	PLoS Genet; The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.; Authors - Deyong Xiao ; KO Validated
human	WB,IHC,IF,IP	Cancer Sci; SMYD4 monomethylates PRMT5 and forms a positive feedback loop to promote hepatocellular carcinoma progression; Authors - Zhenyu Zhou