SMYD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMYD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 12011-1-AP

Synonyms

ZMYND1, Zinc finger MYND domain-containing protein 1, SET and MYND domain-containing protein 3, Histone-lysine N-methyltransferase SMYD3, EC:2.1.1.354

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Validation Data Gallery

• 

  WB analysis of mouse brain using 12011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12011-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12011-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12011-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12011-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12011-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12011-1-AP

  IP result of anti-SMYD3 (IP:12011-1-AP, 4ug; Detection:12011-1-AP 1:500) with COLO 320 cells lysate 1280ug.

• 

  IHC staining of human breast cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12011-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 12011-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SMYD3 antibody (12011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, HeLa cells, HepG2 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

12011-1-AP targets SMYD3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMYD3 fusion protein Ag2624
• Full Name: SET and MYND domain containing 3
• Calculated Molecular Weight: 428aa,49 kDa; 369aa,42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC031010
• Gene Symbol: SMYD3
• Gene ID (NCBI): 64754
• RRID: AB_2193981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) . SMYD3 exists some isoforms with MV 49, 43 and 30 kDa.

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B; SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.; Authors - Fen Long ; KD Validated
human	WB	Carcinogenesis; miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma.; Authors - Furuta Mayuko M
human	WB	Int J Androl; Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.; Authors - Lambrot R R
human	WB	Med Oncol; PMID: 20957523; Authors - Ren Tian-nian TN ; KD Validated
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu
human	WB,IHC	Oncogenesis; SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair; Authors - Yujia Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

It worked well in the HEK293T cells with human SMYD3 overexpression.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: HEK293T cells

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