SMYD1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

SMYD1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 16151-1-AP

Synonyms

BOP, SMYD1, SMYD1-Specific, ZMYND18, ZMYND22

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Validation Data Gallery

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  WB analysis of mouse heart using 16151-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16151-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16151-1-AP

  IP result of anti-SMYD1-Specific (IP:16151-1-AP, 4ug; Detection:16151-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human testis using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue, human lung tissue, human ovary tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16151-1-AP targets SMYD1-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SET and MYND domain containing 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_198274
• Gene Symbol: SMYD1
• Gene ID (NCBI): 150572
• RRID: AB_2239747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Toxicol Lett; Down-regulated Smyd1 participated in the inhibition of myoblast differentiation induced by cigarette smoke extract; Authors - Fang Li ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

SMYD1 antibody worked very well in human differentiated cardiomyocyte.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Human cardiomyocytes

FH

Eric (Verified Customer) (10-15-2018)

Very low detection of SMYD1: 45 ug protein, overnight incubation with primary antibody at 4 oC, 2h incubation with secondary at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: C2C12/mouse heart