SMURF1 Monoclonal antibody, PBS Only

SMURF1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7E4

Cat no : 68466-1-PBS

Synonyms

hSMURF1, KIAA1625, SMURF1

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Validation Data Gallery

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  WB analysis using 68466-1-Ig (same clone as 68466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68466-1-Ig (SMURF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68466-1-PBS in a different storage buffer formulation.

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Product Information

68466-1-PBS targets SMURF1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMURF1 fusion protein Ag30891
• Full Name: SMAD specific E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: NM_020429
• Gene Symbol: SMURF1
• Gene ID (NCBI): 57154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).