SMURF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMURF1 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 55175-1-AP

Synonyms

SMAD-specific E3 ubiquitin-protein ligase 1, KIAA1625, hSMURF1, HECT-type E3 ubiquitin transferase SMURF1, EC:2.3.2.26

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Validation Data Gallery

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  IHC staining of human colon cancer using 55175-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 55175-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 55175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 55175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue, mouse spleen tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

55175-1-AP targets SMURF1 in IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD specific E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_020429
• Gene Symbol: SMURF1
• Gene ID (NCBI): 57154
• RRID: AB_10859079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

Publications

Species	Application	Title
human		J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo
rat		Free Radic Biol Med; CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.; Authors - Wenyan Gong
human	IP,IHC	Clin Transl Med; MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.; Authors - Yuanyuan Chang
human		J Immunol; MLN4924, a First-in-Class NEDD8-Activating Enzyme Inhibitor, Attenuates IFN-β Production.; Authors - Hui Song ; KD Validated
mouse		Biomed Pharmacother; Fucoxanthin rescues dexamethasone induced C2C12 myotubes atrophy.; Authors - Liao Zhiyin
mouse		Ecotoxicol Environ Saf; Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.; Authors - Di Wang