SMUG1 Recombinant antibody

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  WB analysis using 83771-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83771-5-RR (SMUG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of U-2 OS using 83771-5-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug SMUG1 Recombinant antibody (83771-5-RR, Clone:240817E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83771-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83771-5-RR against Human SMUG1 were performed. The affinity constant is below 1 pM.

SMUG1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83771-5-RR

Clone No.240817E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-

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Tested Applications

• Positive WB detected in: MOLT-4 cells, K-562 cells, HEK-293 cells, COLO 320 cells, HeLa cells
• Positive FC (Intra) detected in: U-2 OS

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83771-5-RR targets SMUG1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMUG1 fusion protein Ag13934
• Full Name: single-strand-selective monofunctional uracil-DNA glycosylase 1
• Calculated Molecular Weight: 177 aa, 20 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC000417
• Gene Symbol: SMUG1
• Gene ID (NCBI): 23583
• RRID: AB_3671365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q53HV7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).