SMUG1 Recombinant antibody

SMUG1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83771-5-RR
Clone No.240817E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inMOLT-4 cells, K-562 cells, HEK-293 cells, COLO 320 cells, HeLa cells
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83771-5-RR targets SMUG1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMUG1 fusion protein Ag13934 Predict reactive species
Full Name single-strand-selective monofunctional uracil-DNA glycosylase 1
Calculated Molecular Weight 177 aa, 20 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC000417
Gene Symbol SMUG1
Gene ID (NCBI) 23583
RRIDAB_3671365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ53HV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

Protocols

Product Specific Protocols
WB protocol for SMUG1 antibody 83771-5-RRDownload protocol
Standard Protocols
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