SMUG1 Polyclonal antibody

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  WB analysis using 19857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19857-1-AP (SMUG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SMUG1 Polyclonal Antibody for WB, ELISA

Cat No. 19857-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FDG, HMUDG, SMUG1, UNG3

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells, HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19857-1-AP targets SMUG1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMUG1 fusion protein Ag13934
• Full Name: single-strand-selective monofunctional uracil-DNA glycosylase 1
• Calculated Molecular Weight: 177 aa, 20 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000417
• Gene Symbol: SMUG1
• Gene ID (NCBI): 23583
• RRID: AB_3085589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53HV7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). This antibody is specific to UNG.