Tested Applications
Positive WB detected in | COLO 320 cells, HEK-293 cells, HeLa cells, K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
19857-1-AP targets SMUG1 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SMUG1 fusion protein Ag13934 Predict reactive species |
Full Name | single-strand-selective monofunctional uracil-DNA glycosylase 1 |
Calculated Molecular Weight | 177 aa, 20 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC000417 |
Gene Symbol | SMUG1 |
Gene ID (NCBI) | 23583 |
RRID | AB_3085589 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q53HV7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). This antibody is specific to UNG.
Protocols
Product Specific Protocols | |
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WB protocol for SMUG1 antibody 19857-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |