SMTN Polyclonal antibody

SMTN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 23567-1-AP

Synonyms

smoothelin, SMSMO, SMTN

---

Validation Data Gallery

• 

  WB analysis of COLO 320 using 23567-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23567-1-AP (SMTN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 23567-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23567-1-AP (SMTN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human hysteromyoma using 23567-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 23567-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hysteromyoma using 23567-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 23567-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23567-1-AP (SMTN Antibody) at dilution of 1:200 (under 10x lens).

---

Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IHC detected in: human hysteromyoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

23567-1-AP targets SMTN in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.

• Tested Reactivity: mouse, human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMTN fusion protein Ag19983
• Full Name: smoothelin
• Calculated Molecular Weight: 940 aa, 102 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC034237
• Gene Symbol: SMTN
• Gene ID (NCBI): 6525
• RRID: AB_2879298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMTN, also named as Smoothelin, is a 917 amino acid protein, which contains 1 CH (calponin-homology) domain and belongs to the smoothelin family. SMTN as structural protein of the cytoskeleton involve in muscle organ development and smooth muscle contraction.

Publications

Species	Application	Title
human	WB,IHC	bioRxiv; Coordination between cytoskeletal organization, cell contraction and extracellular matrix development, is depended on LOX for aneurysm prevention; Authors - Rohtem Aviram
mouse	WB	Am J Physiol Renal Physiol; PMID: 31066574; Authors - Nao Iguchi
human	WB	Int J Nanomedicine; Glycosylation-Engineered Platelet Membrane-Coated Interleukin 10 Nanoparticles for Targeted Inhibition of Vascular Restenosis; Authors - Fengshi Li
human,mouse	WB	J Mol Med (Berl); Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation; Authors - Yinghui Chen