SMPD2 Polyclonal antibody

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  WB analysis of mouse liver using 15239-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15239-1-AP (SMPD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SMPD2 Polyclonal Antibody for WB, ELISA

Cat No. 15239-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Lyso PAF PLC, N SMase, Neutral sphingomyelinase, NSMASE, NSMASE1, SMPD2

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15239-1-AP targets SMPD2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMPD2 fusion protein Ag7367
• Full Name: sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000038
• Gene Symbol: SMPD2
• Gene ID (NCBI): 6610
• RRID: AB_3085456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60906
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMPD2, Sphingomyelin phosphodiesterase 2, is also named as Lyso-platelet-activating factor-phospholipase C and neutral sphingomyelinase (nSMase). It is involved in the sphingolipid metabolism pathway, which can catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Smpd2/Smpd3 double-mutant mice were found to have severe retardation of late embryonic and postnatal growth and delayed puberty, belonging to hypothalamus-induced combined pituitary hormone deficiency (PubMed: 15764706).