SMO Polyclonal antibody

SMO Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

38

Reviews (1)

Cat no : 20787-1-AP

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Validation Data Gallery

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  IHC staining of human prostate cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20787-1-AP (SMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20787-1-AP (SMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue, human testis tissue, human colon cancer tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 5 publications below
• IF: See 6 publications below

Product Information

20787-1-AP targets SMO in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMO fusion protein Ag14322
• Full Name: smoothened homolog (Drosophila)
• Calculated Molecular Weight: 787 aa, 86 kDa
• GenBank Accession Number: BC009989
• Gene Symbol: SMO
• Gene ID (NCBI): 6608
• RRID: AB_2878740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays a central role in animal development and stem-cell function. Defects in Hh signaling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Publications

Species	Application	Title
human,mouse		Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
chicken	IF	Sci Adv; Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.; Authors - Gabriela Toro-Tapia
human	IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	IF	J Cell Biol; Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.; Authors - Sarah Findakly
human	IF	Acta Neuropathol Commun; Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals.; Authors - Sarah Findakly
human		Cell Death Discov; N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness; Authors - Hongliang Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (05-13-2019)

For IF, it could label the ciliary localisation of endogenous Smo; for WB, it could label many bands around 100 kd.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF