SMNDC1 Monoclonal antibody, PBS Only

SMNDC1 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1B10C2

Cat no : 68344-1-PBS

Synonyms

Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR, SMN related protein

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Validation Data Gallery

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  WB analysis using 68344-1-Ig (same clone as 68344-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68344-1-Ig (SMNDC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68344-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68344-1-Ig (same clone as 68344-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug SMNDC1 Monoclonal antibody (68344-1-Ig, Clone: 1B10C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68344-1-PBS in a different storage buffer formulation.

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Product Information

68344-1-PBS targets SMNDC1 in Indirect ELISA, WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMNDC1 fusion protein Ag33140
• Full Name: survival motor neuron domain containing 1
• Calculated Molecular Weight: 238 aa, 27 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC011234
• Gene Symbol: SMNDC1
• Gene ID (NCBI): 10285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).