SMN (Human-Specific) Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SMN (Human-Specific) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C6D9

Cat no : 60154-1-PBS

Synonyms

SMN, Gemin-1, Gemin 1, Component of gems 1, C BCD541

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Validation Data Gallery

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  WB analysis using 60154-1-Ig (same clone as 60154-1-PBS)

  A375, HEK-293, and Jurkat cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60154-1-Ig (same clone as 60154-1-PBS)

  WB result of SMN (Human-Specific) antibody (60154-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human-Specific) transfected HEK-293 cells. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 60154-1-Ig (same clone as 60154-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60154-1-Ig (same clone as 60154-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 60154-1-Ig (same clone as 60154-1-PBS)

  IP result of anti-SMN (Human-Specific) (IP:60154-1-Ig, 4ug; Detection:60154-1-Ig 1:500) with HEK-293 cells lysate 2440ug. This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60154-1-Ig (same clone as 60154-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human-Specific) antibody (60154-1-Ig, Clone: 2C6D9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 60154-1-Ig (same clone as 60154-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug SMN (Human-Specific) Monoclonal antibody (60154-1-Ig, Clone: 2C6D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60154-1-PBS in a different storage buffer formulation.

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Product Information

60154-1-PBS targets SMN (Human-Specific) in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMN (Human-Specific) fusion protein Ag14333
• Full Name: survival of motor neuron 2, centromeric
• Calculated Molecular Weight: 282 aa, 30 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000908
• Gene Symbol: SMN
• Gene ID (NCBI): 6607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.