SMN-Exon7 Monoclonal antibody, PBS Only

SMN-Exon7 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A8G11

Cat no : 60255-1-PBS

Synonyms

Component of gems 1, Gemin 1, SMN, SMN1, SMN2, SMNC, SMN-Exon7, SMNT, Survival motor neuron protein, T BCD541

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Validation Data Gallery

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  WB analysis of HEK-293 using 60255-1-Ig (same clone as 60255-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60255-1-Ig (same clone as 60255-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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Product Information

60255-1-PBS targets SMN-Exon7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMN-Exon7 fusion protein Ag16615
• Full Name: survival of motor neuron 1, telomeric
• Calculated Molecular Weight: 294 aa, 32 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC062723
• Gene Symbol: SMN
• Gene ID (NCBI): 6606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.