SMG6 Monoclonal antibody

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  WB analysis using 68109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  WB analysis of RAW 264.7 using 68109-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SMG6 Monoclonal Antibody for WB, ELISA

Cat No. 68109-1-Ig

Clone No.1D2G2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

C17orf31, EST1 like protein A, EST1A, Ever shorter telomeres 1A, hSmg5/7a, KIAA0732, SMG 6, Smg 6 homolog, SMG6, Telomerase subunit EST1A

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Tested Applications

• Positive WB detected in: LNCaP cells, RAW 264.7 cells, MCF-7 cells, HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68109-1-Ig targets SMG6 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMG6 fusion protein Ag30182
• Full Name: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_017575
• Gene Symbol: SMG6
• Gene ID (NCBI): 23293
• RRID: AB_2923638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86US8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMG6, also known as C17orf31, EST1A, and hEST1A, is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini (PMID: 19179534). It promotes in vitro the ability of TERT to elongate telomeres (PMID: 12676087, 12699629). SMG6 also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD (PMID: 19060897, 20930030). Alternate splicing results in multiple transcript variants (PMID: 12699629, 14636577).