SMARCD3 Polyclonal antibody

SMARCD3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 12838-1-AP

Synonyms

Rsc6p, CRACD3, BRG1-associated factor 60C, BRG1 associated factor 60C, BAF60C



Tested Applications

Positive WB detected inHeLa cells, BxPC-3 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman heart tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

12838-1-AP targets SMARCD3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMARCD3 fusion protein Ag3896
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54-55 kDa
GenBank Accession NumberBC002628
Gene Symbol SMARCD3
Gene ID (NCBI) 6604
RRIDAB_2192157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCD3 is a component of the SWI/SNF (mating type switching/sucrose non-fermenting) chromatin remodeling complex, which evolutionarily conserved from yeast to human, and all complexes contain a core set of conserved components, including BRG1/Brm-associated factors (BAFs) and a DNA-dependent SWI2/SNF2-like ATPase, which enables chromatin remodeling. SMARCD3 isoforms bind to several nuclear receptors and transcription factors of various families. SMARCD3 proteins interact in a ligand-independent manner with peroxisome proliferator- activated receptor gamma and enhance its transcriptional activity.

Protocols

Product Specific Protocols
WB protocol for SMARCD3 antibody 12838-1-APDownload protocol
IHC protocol for SMARCD3 antibody 12838-1-APDownload protocol
IF protocol for SMARCD3 antibody 12838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo
mouseWB

Cardiovasc Res

The G protein coupled receptor Apj is expressed in the second heart field and regulates Cerberus-Baf60C axis in embryonic stem cell cardiomyogenesis.

Authors - D'Aniello Cristina C
humanWB

Transl Oncol

SNF5, a core subunit of SWI/SNF complex, regulates melanoma cancer cell growth, metastasis, and immune escape in response to matrix stiffness.

Authors - Ying Chen
humanWB

Cells

The SMARCD Family of SWI/SNF Accessory Proteins Is Involved in the Transcriptional Regulation of Androgen Receptor-Driven Genes and Plays a Role in Various Essential Processes of Prostate Cancer

Authors - Iris E Ertl
mouseWB

Nat Cardiovasc Res

Translational landscape of direct cardiac reprogramming reveals a role of Ybx1 in repressing cardiac fate acquisition

Authors - Yifang Xie