SMARCD1 Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 66116-1-Ig (same clone as 66116-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66116-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66116-1-Ig (same clone as 66116-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66116-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66116-1-Ig (same clone as 66116-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66116-1-PBS in a different storage buffer formulation.

SMARCD1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66116-1-PBS

Clone No.2F2G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

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Product Information

66116-1-PBS targets SMARCD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMARCD1 fusion protein Ag19100
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC009368
• Gene Symbol: SMARCD1
• Gene ID (NCBI): 6602
• RRID: AB_2881515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96GM5
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1), also known as BAF60a (BRG1-associated factor 60A),Rsc6p or CRACD1, is a 476 amino acid protein that localizes to the nucleus and contains one SWIB domain. Expressed ubiquitously with notable expression in liver, brain, muscle, lung, kidney, pancreas and placenta, SMARCD1 functions as a component of the BAF complex and, in conjunction with a variety of other proteins, plays an essential role in chromatin remodeling. In addition, SMARCD1 influences vitamin D-mediated transcriptional activity and is thought to provide a link between the vitamin D receptor (VDR) and SWI/SNF chromatin remodeling complexes. Multiple isoforms of BAF60a exist due to alternative splicing events.