SMARCA4/BRG1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SMARCA4/BRG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21634-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 21634-1-AP (same clone as 21634-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 21634-1-AP (same clone as 21634-1-PBS)

  WB result of SMARCA4/BRG1 antibody (21634-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21634-1-AP (same clone as 21634-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 21634-1-AP (same clone as 21634-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 21634-1-AP (same clone as 21634-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 21634-1-AP (same clone as 21634-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 21634-1-AP (same clone as 21634-1-PBS)

  IP result of anti-SMARCA4/BRG1 (IP:21634-1-AP, 5ug; Detection:21634-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 21634-1-AP (same clone as 21634-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21634-1-AP (same clone as 21634-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (21634-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 21634-1-AP (same clone as 21634-1-PBS)

  Immunofluorescent analysis of Hela cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 21634-1-AP (same clone as 21634-1-PBS)

  Immunofluorescent analysis of 293 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.

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Product Information

21634-1-PBS targets SMARCA4/BRG1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCA4/BRG1 fusion protein Ag16256
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Calculated Molecular Weight: 1647 aa, 185 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC150298
• Gene Symbol: SMARCA4
• Gene ID (NCBI): 6597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.