SMARCA2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

SMARCA2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 26613-1-AP

Synonyms

SMARCA2, EC:3.6.4.-, BRM, BRG1-associated factor 190B, BAF190B

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Validation Data Gallery

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  WB analysis of HeLa using 26613-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26613-1-AP

  WB result of SMARCA2-Specific antibody (26613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA2-Specific transfected HeLa cells.

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  WB analysis of NIH/3T3 using 26613-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 26613-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 26613-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26613-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26613-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 26613-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (26613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, MDA-MB-453s cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• CoIP: See 1 publications below

Product Information

26613-1-AP targets SMARCA2-Specific in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCA2-Specific fusion protein Ag25157
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 180-190 kDa
• GenBank Accession Number: NM_003070
• Gene Symbol: SMARCA2
• Gene ID (NCBI): 6595
• RRID: AB_2880575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Publications

Species	Application	Title
rat	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
human	WB	Oncol Lett; PMID: 30127991; Authors - Xiaoli Xu
mouse	WB	Stem Cells; Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells.; Authors - Debolina Ganguly
mouse	WB	Biochem Biophys Res Commun; Chromatin remodeler BRM is a key mediator of leucine-stimulated mTOR gene transcription in mouse mammary epithelial cells; Authors - Changping Ke
human	CoIP	Nat Commun; Prion-like domain mediated phase separation of ARID1A promotes oncogenic potential of Ewing's sarcoma; Authors - Yong Ryoul Kim