Filter: AllWBIHC

WB analysis using 67052-1-Ig (same clone as 67052-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67052-1-Ig (SMAD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67052-1-Ig (same clone as 67052-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67052-1-Ig (same clone as 67052-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

SMAD5 Monoclonal antibody, PBS Only

SMAD5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67052-1-PBS

Clone No.1A6A4

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67052-1-PBS targets SMAD5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	SMAD5 fusion protein Ag28699 Predict reactive species
Full Name	SMAD family member 5
Calculated Molecular Weight	465 aa, 52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC009682
Gene Symbol	SMAD5
Gene ID (NCBI)	4090
RRID	AB_2882365
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q99717
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.