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SMAD4 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

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Cat No : 61631,61632 61631

Synonyms

SMAD4, SMAD Family Member 4, Deleted In Pancreatic Carcinoma Locus 4, MADH4, Mothers Against Decapentaplegic Homolog 4, DPC4, Mothers Against Decapentaplegic Drosophila Homolog Of 4, Deletion Target In Pancreatic Carcinoma 4, SMAD Mothers Against DPP Homolog 4, MAD Homolog 4, Smad4, JIP, hSMAD4, MYHRS, Mothers Against DPP Homolog 4, MAD Mothers Against Decapentaplegic Homolog 4 (Drosophila), SMAD 4, SMAD Mothers Against DPP Homolog 4 (Drosophila), coactivator, smad2, smad3, polyclonal, pab, antibody, antibodies, western blotting, wb, sample, immunoprecipitation, ip

Validation Data Gallery

  • SMAD4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SMAD4 antibody was used to immunoprecipitate SMAD4 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMAD4 antibody at a dilution of 1:500.

    SMAD4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SMAD4 antibody was used to immunoprecipitate SMAD4 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMAD4 antibody at a dilution of 1:500.

  • SMAD4 antibody (pAb) tested by Western blot. Detection of SMAD4 by Western blot analysis. Nuclear extract of HeLa cells (30 ug) probed with SMAD4 antibody at a dilution of 1:500.

    SMAD4 antibody (pAb) tested by Western blot. Detection of SMAD4 by Western blot analysis. Nuclear extract of HeLa cells (30 ug) probed with SMAD4 antibody at a dilution of 1:500.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human SMAD4.
Full Name SMAD4 antibody (pAb)
Synonyms SMAD4, SMAD Family Member 4, Deleted In Pancreatic Carcinoma Locus 4, MADH4, Mothers Against Decapentaplegic Homolog 4, DPC4, Mothers Against Decapentaplegic Drosophila Homolog Of 4, Deletion Target In Pancreatic Carcinoma 4, SMAD Mothers Against DPP Homolog 4, MAD Homolog 4, Smad4, JIP, hSMAD4, MYHRS, Mothers Against DPP Homolog 4, MAD Mothers Against Decapentaplegic Homolog 4 (Drosophila), SMAD 4, SMAD Mothers Against DPP Homolog 4 (Drosophila), coactivator, smad2, smad3, polyclonal, pab, antibody, antibodies, western blotting, wb, sample, immunoprecipitation, ip
Molecular weight 72 kDa
GenBank accession numberNP_005350
RRIDAB_2793711
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SMAD4 (Mothers Against Decapentaplegic Homolog 4) or common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.