SMAD3 Monoclonal antibody, PBS Only

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  WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66516-1-Ig (same clone as 66516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 66516-1-Ig (same clone as 66516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

SMAD3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66516-1-PBS

Clone No.1F10B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

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Product Information

66516-1-PBS targets SMAD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 3
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: NM_005902
• Gene Symbol: SMAD3
• Gene ID (NCBI): 4088
• RRID: AB_2881879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P84022
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.