SMAD3 Polyclonal antibody

SMAD3 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 51145-1-AP

Synonyms

hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 51145-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51145-1-AP (SMAD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 40x lens).

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Published Applications

• WB: See 11 publications below

Product Information

51145-1-AP targets SMAD3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 3
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC050743
• Gene Symbol: SMAD3
• Gene ID (NCBI): 4088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD family member 3 (SMAD3), also named Mothers against decapentaplegic homolog 3. Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.The observed molecular weight of SMAD3 is about 48 kDa.

Publications

Species	Application	Title
mouse	WB	Int J Biol Macromol; Chitosan-modified dihydromyricetin liposomes promote the repair of liver injury in mice suffering from diabetes mellitus; Authors - Qiteng Ding
mouse	WB	J Ethnopharmacol; Yishen Tongluo formula alleviates diabetic kidney disease through regulating Sirt6/TGF-β1/Smad2/3 pathway and promoting degradation of TGF-β1; Authors - Xiaowei Zhang
human	WB	Biomed Res Int; Multifunctional Drug-Loaded Phase-Change Nanoparticles Inhibit the Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma by Affecting the Activity of Activated Hepatic Stellate Cells; Authors - Xiaomeng Zou
rat	WB	Dis Markers; Pyrrosia petiolosa Extract Ameliorates Ethylene Glycol-Induced Urolithiasis in Rats by Inhibiting Oxidative Stress and Inflammatory Response; Authors - Fangmin Zhou
mouse	WB	Am J Chin Med; Astragaloside I from Astragalus Attenuates Diabetic Kidney Disease by Regulating HDAC3/Klotho/TGF-β1 Loop; Authors - Xiaowei Zhang
rat	WB	Heliyon; Benzylisoquinoline alkaloids inhibit lung fibroblast activation mainly via inhibiting TGF-β1/Smads and ERK1/2 pathway proteins; Authors - Hui-Li Ren