SMAD2 Recombinant antibody, PBS Only

SMAD2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240950F1

Cat no : 83841-4-PBS

Synonyms

JV18 1, JV18, hSMAD2, hMAD-2, hMAD 2

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Validation Data Gallery

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  WB analysis using 83841-4-RR (same clone as 83841-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83841-4-RR (SMAD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83841-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83841-4-RR (same clone as 83841-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD2 antibody (83841-4-RR, Clone: 240950F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83841-4-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 83841-4-RR (same clone as 83841-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMAD2 Recombinant antibody (83841-4-RR, Clone:240950F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83841-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83841-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83841-4-RR against Human SMAD2 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83841-4-PBS is SMAD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMAD2 fusion protein Ag3237
• Full Name: SMAD family member 2
• Calculated Molecular Weight: 467 aa, 52 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC014840
• Gene Symbol: SMAD2
• Gene ID (NCBI): 4087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)