SLIT2 Polyclonal antibody

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  WB analysis using 28730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28730-1-AP (SLIT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SLIT2 Polyclonal Antibody for WB, ELISA

Cat No. 28730-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Slit-2, Slit homolog 2 protein N-product, Slit homolog 2 protein C-product, Slit homolog 2 protein, Slit 2

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28730-1-AP targets SLIT2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLIT2 fusion protein Ag30428
• Full Name: slit homolog 2 (Drosophila)
• Calculated Molecular Weight: 170 kDa
• Observed Molecular Weight: 100 kDa, 200 kDa
• GenBank Accession Number: NM_004787
• Gene Symbol: SLIT2
• Gene ID (NCBI): 9353
• RRID: AB_2935470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94813
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLIT2, also named as SLIL3, is thought to act as a molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2. Slit2 is cleaved into 140 kDa N-terminal (Slit2-N) and 55-60 kDa C-terminal (Slit2-C) fragments, although the uncleaved/full-length form(200) can also be detected.