SLCO2B1 Recombinant antibody

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  WB analysis using 84353-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84353-1-RR (SLCO2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SLCO2B1 Recombinant Antibody for WB, ELISA

Cat No. 84353-1-RR

Clone No.241199F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

OATPRP2, OATPB, OATP2B1, OATP B, KIAA0880

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84353-1-RR targets SLCO2B1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier organic anion transporter family, member 2B1
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 61~74 kDa
• GenBank Accession Number: NM_007256
• Gene Symbol: SLCO2B1
• Gene ID (NCBI): 11309
• RRID: AB_3671891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O94956
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Solute carrier organic anion (SLCO) gene families encode organic anion transport proteins (OATP), which are membrane transporters widely expressed in the human body that influx numerous compounds and drugs including androgens. SLCO2B1 is a member of an organic anion transporter family and has previously been shown to transport a multitude of drugs and steroid hormone conjugates (PMID: 35714613). High expression of SLCO2B1 is known to associate with the resistance to androgen deprivation therapy in prostate cancer (PMID: 29581838). OATP2B1 was insensitive to endoglycosidase H, an enzyme that cleaves only high mannose glycans, as expected for a mature protein undergoing complex glycosylation.