Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83054-5-RR targets OATP1B1/SLCO1B1 in IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | solute carrier organic anion transporter family, member 1B1 |
| Calculated Molecular Weight | 76 kDa |
| GenBank Accession Number | NM_006446 |
| Gene Symbol | SLCO1B1 |
| Gene ID (NCBI) | 10599 |
| RRID | AB_3670780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y6L6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
A membrane-bound sodium-independent organic anion transporter protein (OATP1B1), encoded by the SLCO1B1 gene (Solute Carrier Organic Anion Transporter Family Member 1B1), is a critical hepatic uptake transporter that plays a pivotal role in the pharmacokinetics and pharmacodynamics of numerous endogenous compounds and xenobiotics. OATP1B1 is expressed predominantly on the basolateral membrane of hepatocytes, where it mediates active intracellular hepatic transport of various anionic compounds. Polymorphisms in the gene encoding this protein are associated with impaired transporter function.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OATP1B1/SLCO1B1 antibody 83054-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
