SLC9A9 Monoclonal antibody

SLC9A9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8D9

Cat no : 66577-1-Ig

Synonyms

Na(+)/H(+) exchanger 9, Nbla00118, NHE 9, NHE9, SLC9A9, Sodium/hydrogen exchanger 9

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Validation Data Gallery

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  WB analysis using 66577-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66577-1-Ig (SLC9A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66577-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLC9A9 antibody (66577-1-Ig, Clone: 1B8D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: human kidney tissue, human tonsillitis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66577-1-Ig targets SLC9A9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC9A9 fusion protein Ag4660
• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 9
• Calculated Molecular Weight: 645 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC035779
• Gene Symbol: SLC9A9
• Gene ID (NCBI): 285195
• RRID: AB_2881937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.