SLC9A3R2 Polyclonal antibody

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  WB analysis using 18891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 18891-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC9A3R2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18891-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

NHERF-2, NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, T-47D cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18891-1-AP targets SLC9A3R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC9A3R2 fusion protein Ag13476
• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
• Calculated Molecular Weight: 337 aa, 37 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC106001
• Gene Symbol: SLC9A3R2
• Gene ID (NCBI): 9351
• RRID: AB_3085570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15599
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)