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NHE3 Recombinant antibody, PBS Only (Detector)

NHE3 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240999B6

Cat no : 83832-3-PBS

Synonyms

SLC9A3, NHE-3, 240999B6

Validation Data Gallery

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  • Cytometric bead array standard curve of MP00818-1

    Cytometric bead array standard curve of MP00818-1

    Cytometric bead array standard curve of MP00818-1, SLC9A3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83832-2-PBS. Detection antibody: 83832-3-PBS. Standard: Ag25637. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00818-2

    Cytometric bead array standard curve of MP00818-2

    Cytometric bead array standard curve of MP00818-2, SLC9A3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83832-5-PBS. Detection antibody: 83832-3-PBS. Standard: Ag25637. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83832-3-PBS

    Affinity and Kinetic Characterization of 83832-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83832-3-PBS against Human NHE3 was performed. The affinity constant is 0.495 nM.

Product Information

83832-3-PBS targets NHE3 as part of a matched antibody pair:

MP00818-1: 83832-2-PBS capture and 83832-3-PBS detection (validated in Cytometric bead array)

MP00818-2: 83832-5-PBS capture and 83832-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NHE3 fusion protein Ag25637
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3
Calculated Molecular Weight 834 aa, 93 kDa
GenBank Accession NumberBC101669
Gene Symbol NHE3
Gene ID (NCBI) 6550
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).