SLC7A5 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC7A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 28670-1-AP

Synonyms

D16S469E, CD98LC, CD98 light chain, 4F2LC, 4F2 light chain

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Validation Data Gallery

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  WB analysis using 28670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28670-1-AP (SLC7A5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 28670-1-AP

  WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 28670-1-AP

  WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

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  IHC staining of human lung cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 28670-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SLC7A5 antibody (28670-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HeLa cells, HepG2 cells, MKN-45 cells, SW480 cells
• Positive IHC detected in: human lung cancer tissue, human placenta tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

28670-1-AP targets SLC7A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC7A5 fusion protein Ag29164
• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Calculated Molecular Weight: 507 aa, 55 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC039692
• Gene Symbol: SLC7A5
• Gene ID (NCBI): 8140
• RRID: AB_2918188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

Publications

Species	Application	Title
mouse	WB	J Inherit Metab Dis; Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission; Authors - Sofía Illescas
human	WB	J Exp Clin Cancer Res; CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex; Authors - Qiang Ma
human	IHC,WB	Front Oncol; ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation; Authors - Daqi Jia
human	WB	Sci Adv; Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells; Authors - Dennis J Hsu
human	WB,IHC	J Exp Clin Cancer Res; FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer; Authors - Zhiyuan Zhou
mouse	WB	Nat Cell Biol; A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments; Authors - Christopher Chidley

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xie (Verified Customer) (07-07-2023)

The expression of Slc7a5 is good for my samples

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: brain

SLC7A5 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:28670-1-AP)