SLC7A4 Polyclonal antibody

SLC7A4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15748-1-AP

Synonyms

VH, HCAT3, CAT-4, CAT4, CAT 4

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Validation Data Gallery

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  WB analysis of mouse brain using 15748-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15748-1-AP (SLC7A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15748-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15748-1-AP (SLC7A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15748-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15748-1-AP (SLC7A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15748-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15748-1-AP (SLC7A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15748-1-AP

  Immunofluorescent analysis of Hela cells, using SLC7A4 antibody 15748-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human liver tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

15748-1-AP targets SLC7A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC7A4 fusion protein Ag8211
• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 4
• Calculated Molecular Weight: 635 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC008814
• Gene Symbol: SLC7A4
• Gene ID (NCBI): 6545
• RRID: AB_2190966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; The Identification of the Metabolism Subtypes of Skin Cutaneous Melanoma Associated With the Tumor Microenvironment and the Immunotherapy.; Authors - Ronghua Yang