SLC7A11/xCT Polyclonal antibody

• 

  WB analysis using 32384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32384-1-AP (SLC7A11/xCT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 32384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32384-1-AP (SLC7A11/xCT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse eye using 32384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using SLC7A11/xCT antibody (32384-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 32384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using SLC7A11/xCT antibody (32384-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC7A11/xCT Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 32384-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

sut, Subtle gray protein, Solute carrier family 7 member 11, Slc7a11, Cystine/glutamate transporter

---

Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells, HepG2 cells, rat brain tissue, mouse kidney tissue, mouse stomach tissue, rat liver tissue
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32384-1-AP targets SLC7A11/xCT in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC7A11/xCT fusion protein Ag38215
• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 70 kDa, 45 kDa
• GenBank Accession Number: NM_011990
• Gene Symbol: Slc7a11
• Gene ID (NCBI): 26570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9WTR6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC7A11 (also known as xCT) is a membrane transporter involved in cysteine uptake. It promotes glutathione synthesis through cystine uptake and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers, including lung cancer. Although different from the predicted size of 55 kDa, the 45- and 70-kDa bands represent xCT, most likely a monomer and homodimer of the protein (PMID: 16399997).