SLC5A8 Polyclonal antibody

SLC5A8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29827-1-AP

Synonyms

AIT, Apical iodide transporter, SLC5A8, SMCT, SMCT1

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Validation Data Gallery

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  WB analysis using 29827-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29827-1-AP (SLC5A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 29827-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 29827-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29827-1-AP targets SLC5A8 in WB, IHC, ELISA applications and shows reactivity with Human, mouse , rat samples.

• Tested Reactivity: Human, mouse , rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC5A8 fusion protein Ag30183
• Full Name: solute carrier family 5 (iodide transporter), member 8
• Calculated Molecular Weight: 610 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC110492
• Gene Symbol: SLC5A8
• Gene ID (NCBI): 160728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 69-kDa protein functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in the healthy human, rat, and mouse colon. And the protein is localized to apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.