SLC5A8 Polyclonal antibody

SLC5A8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 21433-1-AP

Synonyms

Sodium-coupled monocarboxylate transporter 1, Sodium iodide-related cotransporter, SMCT1, SMCT, Apical iodide transporter

---

Validation Data Gallery

• 

  WB analysis using 21433-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21433-1-AP (SLC5A8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21433-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21433-1-AP (SLC5A8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 21433-1-AP

  Immunofluorescent analysis of Hela cells, using SLC5A8 antibody 21433-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

21433-1-AP targets SLC5A8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC5A8 fusion protein Ag16041
• Full Name: solute carrier family 5 (iodide transporter), member 8
• Calculated Molecular Weight: 610 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC110492
• Gene Symbol: SLC5A8
• Gene ID (NCBI): 160728
• RRID: AB_10755299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,IF	Cell Metab; The probiotic L. casei Zhang slows the progression of acute and chronic kidney disease.; Authors - Han Zhu
human,rat	WB	J Ethnopharmacol; Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis; Authors - Hui-Hui Guo
mouse	WB,IHC	Chem Biol Interact; Downregulation of OATP2B1 by proinflammatory cytokines leads to 5-ASA hyposensitivity in Ulcerative colitis; Authors - Ping Li
human	WB	Exp Mol Med; Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury.; Authors - Jing Xiao
human	WB	Front Pharmacol; Ramulus Mori (Sangzhi) Alkaloids (SZ-A) Ameliorate Glucose Metabolism Accompanied by the Modulation of Gut Microbiota and Ileal Inflammatory Damage in Type 2 Diabetic KKAy Mice.; Authors - Quan Liu
mouse	IHC	Sci Rep; Gut-derived butyrate suppresses ocular surface inflammation.; Authors - Laura Schaefer