SLC5A6 Polyclonal antibody

SLC5A6 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26407-1-AP

Synonyms

SLC5A6, SMVT

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Validation Data Gallery

• 

  WB analysis of Caco-2 using 26407-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26407-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 26407-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 26407-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of COLO 320 using 26407-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26407-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 26407-1-AP

  IP result of anti-SLC5A6 (IP:26407-1-AP, 4ug; Detection:26407-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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Tested Applications

• Positive WB detected in: Caco-2 cells, mouse brain tissue, mouse small intestine tissue, COLO 320 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 26407-1-AP is SLC5A6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC5A6 fusion protein Ag23907
• Full Name: solute carrier family 5 (sodium-dependent vitamin transporter), member 6
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC012806
• Gene Symbol: SLC5A6
• Gene ID (NCBI): 8884
• RRID: AB_2880502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Nat Metab; Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer; Authors - Peter Kreuzaler
human	WB	Mol Cell Proteomics; Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.; Authors - James R Byrnes