SLC5A11 Polyclonal antibody

SLC5A11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14089-1-AP

Synonyms

KST1, RKST1, SGLT6, SLC5A11, SLGTX, SMIT2

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Validation Data Gallery

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  WB analysis using 14089-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 14089-1-AP (SLC5A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: C2C12 cells
• Positive IHC detected in: human colon cancer tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14089-1-AP targets SLC5A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC5A11 fusion protein Ag5220
• Full Name: solute carrier family 5 (sodium/glucose cotransporter), member 11
• Calculated Molecular Weight: 611 aa, 67 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC057780
• Gene Symbol: SLC5A11
• Gene ID (NCBI): 115584
• RRID: AB_2189540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Clin Endocrinol Metab; Placental Inositol Reduced in Gestational Diabetes as Glucose Alters Inositol Transporters and IMPA1 Enzyme Expression.; Authors - Reshma A Pillai
human	WB	Front Pharmacol; Quercetin Attenuates Atherosclerosis via Modulating Oxidized LDL-Induced Endothelial Cellular Senescence.; Authors - Yue-Hua Jiang