Band 3/AE 1 Recombinant antibody

Band 3/AE 1 Uni-rAb^TM Recombinant Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3K21

Cat no : 81066-1-RR

Synonyms

Band 3 / AE1, band 3, band 3/ AE1, SLC4A1, Anion exchanger 1

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Validation Data Gallery

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  IHC staining of human placenta using 81066-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 81066-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 81066-1-RR

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 81066-1-RR

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  Affinity and Kinetic Characterization of 81066-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81066-1-RR against Human Band 3/AE 1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81066-1-RR targets Band 3/AE 1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Band 3/AE 1 fusion protein Ag27878
• Full Name: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Calculated Molecular Weight: 911 aa, 102 kDa
• GenBank Accession Number: BC101570
• Gene Symbol: band 3
• Gene ID (NCBI): 6521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.