Band 3/AE 1 Polyclonal antibody

Band 3/AE 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 28131-1-AP

Synonyms

band 3, band 3/ AE1, SLC4A1, Band 3 anion transport protein, Anion exchanger 1

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 28131-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 28131-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28131-1-AP (SLC4A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28131-1-AP (band 3/ AE1antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 28131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (28131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 28131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (28131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: COLO 320 cells, mouse liver tissue
• Positive IHC detected in: human spleen tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

28131-1-AP targets Band 3/AE 1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Band 3/AE 1 fusion protein Ag27878
• Full Name: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Calculated Molecular Weight: 911 aa, 102 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC101570
• Gene Symbol: band 3
• Gene ID (NCBI): 6521
• RRID: AB_2881069
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

Publications

Species	Application	Title
human	WB	Mol Cell; The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.; Authors - Eunus S Ali
human	IP	Int J Hematol; Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.; Authors - Akihito Kitao
mouse	IF	eLife; Piezo1 as a force-through-membrane sensor in red blood cells; Authors - George Vaisey
human	IF	NPJ Regen Med; Ferric citrate and apo-transferrin enable erythroblast maturation with β-globin from hemogenic endothelium; Authors - Soo-Been Jeon
mouse	IF	Elife; High-altitude hypoxia exposure inhibits erythrophagocytosis by inducing macrophage ferroptosis in the spleen; Authors - Wan-Ping Yang
mouse	IHC	Neuron; Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier; Authors - Yuan Xie