SLC43A3 Polyclonal antibody

SLC43A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20131-1-AP

Synonyms

Protein FOAP 13, PRO1659, FOAP 13, Equilibrative nucleobase transporter 1, ENBT1

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Validation Data Gallery

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  WB analysis using 20131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20131-1-AP (SLC43A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 20131-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20131-1-AP (SLC43A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 20131-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20131-1-AP (SLC43A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, MCF-7 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20131-1-AP targets SLC43A3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC43A3 fusion protein Ag13999
• Full Name: solute carrier family 43, member 3
• Calculated Molecular Weight: 491 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC003163
• Gene Symbol: SLC43A3
• Gene ID (NCBI): 29015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sodium-independent purine-selective nucleobase transporter(SLC43A3), also called as equilibrative nucleobase transporter 1(ENBT1), is a nucleobase transporter involved in purine salvage pathways in mammals, which mediates the equilibrative transport of extracellular purine nucleobases such as adenine, guanine and hypoxanthine.(PubMed:26455426, PubMed:32339528) It has been found that it may regulate the transport of fatty acids (FA) in fat cells, as a positive regulator of FA efflux and as a negative regulator of FA uptake.(PMID: 32217606)