SLC39A5 Polyclonal antibody

SLC39A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17285-1-AP

Synonyms

ZIP-5, ZIP5, ZIP 5, MYP24, LZT-Hs7

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Validation Data Gallery

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  WB analysis using 17285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17285-1-AP (SLC39A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 17285-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17285-1-AP (SLC39A5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17285-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17285-1-AP (SLC39A5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, rat kidney tissue, rat liver tissue, rat pancreas tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17285-1-AP targets SLC39A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC39A5 fusion protein Ag11067
• Full Name: solute carrier family 39 (metal ion transporter), member 5
• Calculated Molecular Weight: 539 aa, 56 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC027884
• Gene Symbol: SLC39A5
• Gene ID (NCBI): 283375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC39A5 (Zip5) belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Most of ZIP members have eight predicted transmembrane domains and similar predicted topologies with the N- and C-termini of the protein located on the extracytoplasmic face of the membrane. Zip5 is a zinc uptake transporter that is specific for Zn(II) over other potential metal ion substrates. ZIP5 gene is most actively expressed in tissues involved in zinc homeostasis (intestine, visceral endoderm, pancreas) but is not induced during zinc deficiency. ZIP5 is localized to the basolateral surface of these cells under zinc-replete conditions but is internalized during periods of dietary zinc deficiency. These observations suggest that Zip5 plays a central role in controlling organismal zinc status. This antibody was generated against the N-terminal region of human SLC39A5 and is predicted to detect the endogenous level of SLC39A5 protein. The calculated molecular weight of SLC39A5 is 56 kDa. With glycosylation modification, the molecular weight of SLC39A5 will be migrated to 70 kDa.

Publications

Species	Application	Title
	WB	Animals (Basel); Analysis of the Association of Two SNPs in the Promoter Regions of the PPP2R5C and SLC39A5 Genes with Litter Size in Yunshang Black Goats; Authors - Peng Wang