SLC39A2 Polyclonal antibody

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  WB analysis using 21287-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21287-1-AP (SLC39A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21287-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21287-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21287-1-AP (SLC39A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21287-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SLC39A2 antibody (21287-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SLC39A2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21287-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ZIP-2, ZIP2, hZIP2, Eti-1, Eti 1

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21287-1-AP targets SLC39A2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC39A2 fusion protein Ag15807
• Full Name: solute carrier family 39 (zinc transporter), member 2
• Calculated Molecular Weight: 309 aa, 33 kDa
• Observed Molecular Weight: 33 kDa, 66-70 kDa
• GenBank Accession Number: BC096723
• Gene Symbol: SLC39A2
• Gene ID (NCBI): 29986
• RRID: AB_3085650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NP94
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC39A2 (hZIP2) is a human zinc importer in the ZIP family, which is essential in the maintenance of intracellular zinc homeostasis and is upregulated by zinc depletion. SLC39A2 is highly expressed in prostate epithelial cells and is involved in prostate cancer development. Most SLC39 proteins consist of eight transmembrane domains (TMDs), with extracellular or intravesicular amino and carboxy termini (PMID: 34790705). Zip2 gene knockout exacerbated myocardial I/R injury, whereas Zip2 gene transfer reduced myocardial infarction (PMID: 31095941). The predicted molecular weight of SLC39A2 is 33 kDa, and a band of dimers at 66 kDa can also be detected by this antibody (PMID: 24619115).