SLC39A14/ZIP-14 Polyclonal antibody

SLC39A14/ZIP-14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26540-1-AP

Synonyms

cig19, KIAA0062, LZT Hs4, SLC39A14, Zinc transporter ZIP14, ZIP 14, ZIP14, Zrt and Irt like protein 14

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Validation Data Gallery

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  WB analysis using 26540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26540-1-AP (SLC39A14/ZIP-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 26540-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26540-1-AP (SLC39A14/ZIP-14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 26540-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SLC39A14/ZIP-14 antibody (26540-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse spleen tissue, rat heart tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

26540-1-AP targets SLC39A14/ZIP-14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC39A14/ZIP-14 fusion protein Ag24210
• Full Name: solute carrier family 39 (zinc transporter), member 14
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC015770
• Gene Symbol: SLC39A14/ZIP-14
• Gene ID (NCBI): 23516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Metal cation symporter ZIP14 (SLC39A14) is an electroneutral transporter of the plasma membrane that mediates the cellular uptake of divalent metal cations zinc, manganese, and iron and is important for tissue homeostasis, metabolism, development and immunity (PubMed:15642354, PubMed:27231142, PubMed:29621230). The molecular weight is around 53-54 kDa.

Publications

Species	Application	Title
human	IHC	Front Mol Biosci; Single-cell transcriptomics uncover the key ferroptosis regulators contribute to cancer progression in head and neck squamous cell carcinoma; Authors - Fei Liu
human	WB	Arthritis Res Ther; Identification and verification of a novel signature that combines cuproptosis-related genes with ferroptosis-related genes in osteoarthritis using bioinformatics analysis and experimental validation; Authors - Baoqiang He
human	WB	Adv Sci (Weinh); Creatine Promotes Endometriosis by Inducing Ferroptosis Resistance via Suppression of PrP; Authors - Siman Chen
mouse	WB,IHC	Cell Signal; Elucidating ferroptosis mechanisms in heart failure through transcriptomics, single-cell sequencing, and experimental validation; Authors - Kaiyuan Li