SLC38A7 Recombinant antibody

SLC38A7 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240147C3

Cat no : 83346-6-RR

Synonyms

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

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Validation Data Gallery

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  WB analysis of Caco-2 using 83346-6-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 83346-6-RR

  human liver tissue were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Caco-2 using 83346-6-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of U-251 using 83346-6-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83346-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83346-6-RR against Human SLC38A7 were performed. The affinity constant is 1.31 nM.

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Tested Applications

• Positive WB detected in: Caco-2 cells, human liver tissue
• Positive FC (Intra) detected in: Caco-2 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83346-6-RR is SLC38A7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC38A7 fusion protein Ag35081
• Full Name: solute carrier family 38, member 7
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001961
• Gene Symbol: SLC38A7
• Gene ID (NCBI): 55238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC38A7 also known as SNAT7, is a member of the SLC38 family that encodes sodium-coupled neutral amino acid transporters. SLC38A7 is a system N transporter that has a substrate preference for L-glutamine. It also transports other amino acids with polar side chains, as well as L-histidine and L-alanine (PMID: 21511949).