SLC38A7 Polyclonal antibody

SLC38A7 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 31486-1-AP

Synonyms

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

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Validation Data Gallery

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  IHC staining of mouse brain using 31486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31486-1-AP (SLC38A7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31486-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31486-1-AP (SLC38A7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 31486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC38A7 antibody (31486-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31486-1-AP targets SLC38A7 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC38A7 fusion protein Ag35081
• Full Name: solute carrier family 38, member 7
• Calculated Molecular Weight: 50 kDa
• GenBank Accession Number: BC001961
• Gene Symbol: SLC38A7
• Gene ID (NCBI): 55238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC38A7 also known as SNAT7, is a system N transporter with a substrate preference for L-glutamine(PMID: 21511949). SLC38A7 symporter that selectively transports sodium ions and amino acids, such as L-glutamine and L-asparagine from the lysosome into the cytoplasm and may participate in mTORC1 activation (PubMed:28416685, PubMed:35561222). colocalization of Slc38a7 with neuronal markers in excitatory and inhibitory neurons, but not in astrocytes or glial cells.