SLC38A6 Polyclonal antibody

SLC38A6 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27636-1-AP

Synonyms

SNAT6, N-system amino acid transporter 1, NAT-1, NAT1, Amino acid transporter SLC38A6

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 27636-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27636-1-AP (SLC38A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 27636-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27636-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 27636-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC38A6 antibody (27636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27636-1-AP targets SLC38A6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC38A6 fusion protein Ag24654
• Full Name: solute carrier family 38, member 6
• Calculated Molecular Weight: 456 aa, 51 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC110378
• Gene Symbol: SLC38A6
• Gene ID (NCBI): 145389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC38A6 is also known as SNAT6 and belongs to the amino acid/polyamine transporter 2 family. SLC38A6 is probably a sodium-dependent amino acid/proton antiporter or a neuronal transporter for glutamate. SLC38A6 has 2 isoforms with the molecular mass of 51 and 58 kDa. The observed molecular weight of SLC38A6 is 43 kDa, which is consistent with the description in the literature of MW between 37 and 50 kDa (PMID: 24752331).