• Featured Product
  • KD/KO Validated

SLC38A3 Polyclonal antibody

SLC38A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14315-1-AP

Synonyms

SNAT3, SN1, N-system amino acid transporter 1, NAT1, Na(+)-coupled neutral amino acid transporter 3



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse pancreas tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14315-1-AP targets SLC38A3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC38A3 fusion protein Ag5553
Full Name solute carrier family 38, member 3
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC042875
Gene Symbol SLC38A3
Gene ID (NCBI) 10991
RRIDAB_2254879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.

Protocols

Product Specific Protocols
WB protocol for SLC38A3 antibody 14315-1-APDownload protocol
IHC protocol for SLC38A3 antibody 14315-1-APDownload protocol
IF protocol for SLC38A3 antibody 14315-1-APDownload protocol
IP protocol for SLC38A3 antibody 14315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Environ Pollut

Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions

Authors - Jae Soon Kang
mouseWB,IF,CoIP

Int J Mol Sci

The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia.

Authors - Katarzyna Dąbrowska
mouseIHC

Cancer Lett

Amino acid transporter SLC38A3 promotes metastasis of non-small cell lung cancer cells by activating PDK1.

Authors - Yanhui Wang
humanWB

Cancer Lett

RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC cells.

Authors - Junyu Wu
mouseIF

iScience

Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1-/- mice

Authors - Jing Wang
mouseWB

Int J Mol Sci

Sex Modulates Response to Renal-Tubule-Targeted Insulin Receptor Deletion in Mice

Authors - Soha Sohail