SLC37A4 Polyclonal antibody

SLC37A4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20612-1-AP

Synonyms

G6PT, G6PT1, G6PT2, G6PT3, GSD1b, GSD1c, GSD1d, PRO0685, SLC37A4, TRG 19, TRG19

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Validation Data Gallery

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  WB analysis of mouse liver using 20612-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20612-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20612-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, HepG2 cells, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 20612-1-AP is SLC37A4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC37A4 fusion protein Ag14660
• Full Name: solute carrier family 37 (glucose-6-phosphate transporter), member 4
• Calculated Molecular Weight: 429 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC015650
• Gene Symbol: SLC37A4
• Gene ID (NCBI): 2542
• RRID: AB_10858386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Discov; Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p; Authors - Jianye Zhong