SLC31A1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SLC31A1 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4H5

Cat no : 67221-1-PBS

Synonyms

Copper transporter 1, COPT1, CTR1, hCTR1, SLC31A1

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Validation Data Gallery

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  WB analysis of HepG2 using 67221-1-Ig (same clone as 67221-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67221-1-Ig (SLC31A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67221-1-Ig (same clone as 67221-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67221-1-Ig (SLC31A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67221-1-Ig (same clone as 67221-1-PBS)

  WB result of SLC31A1 antibody (67221-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells. This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67221-1-Ig (same clone as 67221-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67221-1-Ig (same clone as 67221-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC31A1 (67221-1-Ig, Clone:1A4H5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67221-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67221-1-PBS targets SLC31A1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC31A1 fusion protein Ag28410
• Full Name: solute carrier family 31 (copper transporters), member 1
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC013611
• Gene Symbol: SLC31A1
• Gene ID (NCBI): 1317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.