SLC31A1 Polyclonal antibody

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• KD/KO Validated

SLC31A1 Polyclonal Antibody for WB, IF-P, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27499-1-AP

Synonyms

COPT1, Truncated CTR1 form, CTR1

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Validation Data Gallery

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  WB analysis of HT-29 using 27499-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27499-1-AP

  WB result of SLC31A1 antibody (27499-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

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  WB analysis using 27499-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of human liver cancer using 27499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SLC31A1 antibody (27499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 27499-1-AP

  1X10^6 HeLa cells were surface stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Tested Applications

• Positive WB detected in: HT-29 cells, Caco-2 cells, HeLa cells
• Positive IF-P detected in: human liver cancer tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

27499-1-AP targets SLC31A1 in WB, IHC, IF-P, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC31A1 fusion protein Ag26601
• Full Name: solute carrier family 31 (copper transporters), member 1
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21-25,30-35,60-70 kDa
• GenBank Accession Number: BC013611
• Gene Symbol: SLC31A1
• Gene ID (NCBI): 1317
• RRID: AB_2918124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC31A1, also known as CTR1, COPT1 and solute carrier family 31 member 1, is a high-affinity copper transporter found in the cell membrane of human cells. It plays a crucial role in maintaining copper homeostasis within the body and functions as a homotrimer to influence dietary copper absorption in the cell membrane (PMID: 37853210; 28507097). SLC31A1 has three putative transmembrane domains, with an N-terminus located extracellularly and a C-terminus located intracellularly (PMID: 12827356). SLC31A1 can be detected a a 28 kDa band corresponding to glycosylated hCTR1 monomer, and a 24 kDa band that is unglycosylated hCTR1. In some membrane preparations a C-terminal degradation product of 17 kDa is also seen (PMID: 16135512). Additionally, SLC31A1 may also resolve as a 30 to 35 kDa and a 60 to 70 kDa protein on immunoblots, forming oligomers corresponding to the expected size of a homotrimeric complex (PMID: 12827356).

Publications

Species	Application	Title
	WB	Int J Mol Sci; Transcriptional Responses of Copper-Transport-Related Genes ctr1, ctr2 and atox1 and Their Roles in the Regulation of Cu Homeostasis in Yellow Catfish Pelteobagrus fulvidraco; Authors - Hong Yang
human	WB	Cancer Res Commun; Targeting NEDDylation is a Novel Strategy to Attenuate Cisplatin-induced Nephrotoxicity; Authors - Trace M Jones
mouse	WB,IF	Gene; The STAT1-SLC31A1 axis: Potential regulation of cuproptosis in diabetic retinopathy; Authors - Qiang Hu
human	WB,IHC	J Assist Reprod Genet; Blocking lactate regulation of the Grhl2/SLC31A1 axis inhibits trophoblast cuproptosis and preeclampsia development; Authors - Xue-Yan Shen